7 For genotyping, we used a multilocus allele-specific hybridization assay developed by Roche Molecular Systems (RMS), Alameda, as previously described (some included genes changed from the original description). Genomic DNA was isolated from newborn blood specimens as described elsewhere. Only one polymorphism revealed genotype distributions not statistically consistent with Hardy-Weinberg expectations, ADRB2 (Gln27Glu). Genotypes among these infants were tested to verify that their distributions fit Hardy-Weinberg expectations. All interviews and samples were obtained with approval from the State of California Health and Welfare Agency Committee for the Protection of Human Subjects. Of the 488 infants whose mothers were interviewed, a blood specimen was obtained and genotyped for 437. Analyses in the current study were restricted to infants who were live-born because the source of DNA was residual newborn screening blood specimens. Interviews were completed with 488 (75%) mothers. These infants had no major structural congenital anomalies identified before the first birthday. A total of 652 infants were randomly selected from this population cohort. To ascertain cases, we searched the Radiology Information System at our institution for the text strings “infarct,” “thrombosis,” “venous sinus,” and “stroke” among all head magnetic resonance imaging and computed tomography scans performed from 1989 through 2000 in children 20 weeks gestation that occurred between January 1987 and December 1988 (n=344 214) in selected California counties. The candidate genes examined have been associated with the development or progression of cardiovascular disease, including stroke, in adults, but have not all been investigated in the newborn. The objective of this study was to explore frequencies of polymorphisms in candidate genes regulating thrombosis and thrombolysis, nitric oxide, cytokines, hypertension, and cell adhesion in newborns with stroke relative to randomly selected newborns without stroke from the general population. Pathways involved in the pathophysiology of neonatal stroke are diverse and may include thrombosis and thrombolysis, vascular reactivity, inflammation, and cell signaling. 2,3 Yet, many infants with neonatal stroke follow an uncomplicated pregnancy and delivery without identifiable risk factors. 2 Disorders predisposing to neonatal stroke include maternal infertility, preeclampsia, prolonged rupture of membranes, and chorioamnionitis as well as neonatal prothrombotic disorders and infection. The causes of neonatal stroke are likely multifactorial. 1 Two types of strokes occur in the newborn period: arterial ischemic and venous thrombotic. Neonatal stroke is increasingly recognized with an estimated incidence of one in 4000 live births per year. Further studies are needed to determine the interaction of genetic polymorphisms with environmental risk factors in the pathogenesis of neonatal stroke. Results- Of the 31 polymorphisms evaluated, no variant allele was significantly more common than the reference allele in newborns with stroke than in the general population.Ĭonclusions- Using a series of polymorphisms in pathways implicated in the etiology of stroke, newborns with stroke were not distinguished from a normal control group. Methods- We compared frequencies of polymorphisms in genes regulating thrombosis and thrombolysis, nitric oxide, cytokines, vascular tone, and cell adhesion in a hospital-based cohort of 59 newborns with stroke relative to a random sample of 437 California newborns. Pathways involved in the pathophysiology of neonatal stroke are diverse and may include thrombosis and thrombolysis, vascular reactivity, and inflammation. Customer Service and Ordering Informationīackground and Purpose- Neonatal stroke is increasingly recognized with an estimated incidence of one in 4000 live births per year.Stroke: Vascular and Interventional Neurology.Journal of the American Heart Association (JAHA).Circ: Cardiovascular Quality & Outcomes. ![]() Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |